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Hello everyone,
I wanted to use the model checkpoint to predict some of my mgf files. However I don't want to use every PTM where the model was trained on and exclude some. I changed the config fil…
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Hello,
I am using Canu for the first time, I am trying to assemble a fish genome (de novo) in a grid. However it keeps giving me the error rc=256. First I used canu inside a singularity container an…
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Hi,
I'm running freebayes for de novo variant calling on Oxford Nanopore Technology sequencing data (bam files produced by PromethION) for pool sequenced experiment.
I'm testing freebayes on a ch…
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Hi,
I'm currently working on trimming PacBio fastq data and have encountered some challenges with finding the optimal configuration settings for Trim Galore. Initially, my configuration was as foll…
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I'd like to request a term for microcephaly, short stature, and limb abnormalities.
OMIM: https://omim.org/entry/617604. DONSON is also associated with a more severe phenotype, https://www.omim.org…
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Hi,
I have been working on trio-binning of hybrid genome using PacBio long read sequencing. I YAK paternal and maternal genome and assembled de novo genome ~400 Mb using HIFIASM "pat.mat.asm.fas".…
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Hi,
I have been working on trio-binning of hybrid genome using PacBio long read sequencing. I YAK paternal and maternal genome and assembled de novo genome ~400 Mb using HIFIASM "pat.mat.asm.fas". …
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Dear Florian, I have a PhD student that is analysing ddRADSeq data obtained using poolseq strategy for sequencing.
He used stacks for de novo-assembly, exported the consensus sequences of each locus …
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Hi, I am finding some troubles with inspector-correct.
After the last update of scripts, 4 days ago, now it is successfully producing the `contig_corrected.fasta` file. But when looking at the log o…
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Hello,
I'm currently trying to use dada2 to analyze some pacbio hifi amplicon data that is for near full rRNA operons (~ 4kb). I have a few questions about the optimal way to run dada2 for my data…