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Basecalling 후에 통계 확인하는 절차입니다.
`NanoStat` 함수가 없어서 막혀 있는 상황이에요.
생략하고 `NanoFIlt` 도 해봤는데 안됐습니다.
`cmkim@leafeon:/leafeon/analysis1/cmkim/abalone_gut_ont$ NanoStat --fastq barcod17.fastq
NanoStat: c…
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# Issue Report
## Please describe the issue:
The `sequencing_telemetry.js` file is not generated by Dorado. However, this file can still be generated using MinKNOW (Start > Analysis mode > Basec…
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Hi Shian,
The latest dorado basecalling models, e.g. dna_r10.4.1_e8.2_400bps_hac@v4.3.0, only support combined 5mCG_5hmCG or 5mC_5hmC for modified basecalling, not just 5mCG as before. However, the…
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### Ask away!
If I understand correctly, `dorado` now allows for duplex basecalling with modified bases. Will this be supported in this workflow as well? If so, do you have an estimated timeline?
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### Description of the bug
I just want to use the pipeline for QC'ing my nanopore data, but it prematurely terminates after the initial step of the pipeline:
```bash
[70/93224f] process > NFCOR…
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Hi Mohit,
Thanks for developing NanoPsipy. I download the fast5 files under the accession number PRJNA961708. The sizes of BE2C_shGFP_1, BE2C_shGFP_2, BE2C_shPUS7_1, BE2C_shPUS7_2 fast5 fiiles are 19…
ssscj updated
2 months ago
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Hi,
I'm trying to use your codes to run m6anet. During filter basecalling failed reads, i got some errors.
#filter basecalling failed reads:
cd $wt_basecalled_fast5
mkdir workspace_fail
awk '…
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# Issue Report
A subset of my pod5 files of a specific run take 6x longer to basecall than normally / the other files. This was a promethION run and didn't encounter on another run so far.
## De…
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Hello,
I am writing to you because I am having trouble understanding the output of my results. I have performed a RNA sequencing with RNA004 and I want to know which sites are m6A. When I get the t…
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Hi,
I am working on a sequencing run that was created with Gridion. The basecalling option was enabled in MinKNOW during sequencing.
Recently I tried to re-basecall the run using dorado version …