-
Sequence consensus analysis for enzyme that have a binding pocket for compound X (e.g. for ATP, which is known already)
-
Hi,
I collapsed amplicon data and got some truncated reads during the collapse step.
The data was paired-ed reads which were stiched to single reads. The stiched reads were collapsed with the UMI …
-
### Ask away!
I am running the wf-amplicon on PCR amplicons covering a complete virus genome (4 overlapping long amplicons per sample, pooled in one barcode) and map the reads to a reference genome o…
-
Dear Wayne,
Every now and then I'm looking for freely available molecular biology software, e.g. to give it to our beginner students or use it by myself.
For my own little problems I'm actually u…
-
Implement the `HeaviestBundle` algorithm described in Lee _et al._ (2003) to generate consensus sequences for a POA graph.
Can also be used to obtain RNA isoforms.
-
Hi Yan,
I use abPOA to generate a consensus from four sequences. Here is the distribtuion of n_seq / n_cov in each base in the consensus sequence. My question is, is there a way to remove low cover…
-
Hi there!
I was wondering if there's any chance to download a consensus sequence from icarus viewer. I made a de-novo assembly, and I'm running quast with a reference file.
Maybe there's a way to…
-
Hello Trust4 Team,
Thank you for developing such a useful tool.
I’ve encountered an issue and don’t know how to deal with it.
I have 8 bulk RNA-seq samples, and their FASTQ files are approximate…
-
Dear developers:
openDBA has many applications, which are very interesting. Nanopore provides a kmer model for generating standard nanopore signals. By providing a sequence based on this kmer model, …
-
I aligned 38 sequences, average length 72, but including one outlier sequence with a length of 505 and C-terminal homology.
Same thing happens with muscle or belvu. Alignment is long , but I expe…