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Hi there,
Thank you for developing fastp.
I try to run it on a 3'seq experiment (without any option for polyA trimming), and fastp i) does not detect any adapters, ii) does not trim any reads.
And …
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**What is this request referring to?**
polyA signal sequence variant
**What is the name you would like SO to give the term?**
polyA_signal_sequence_variant
**What is the definition that you wo…
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Dear developers,
First of all, I want to say that I am very happy with dorado and really impressed by the quality and the amount of code written for this software.
Recently, as I plan to use do…
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I have a run with multiple barcoded samples that I have demultiplexed and am attempting to run nanopolish polya on. I am using both the "passed" fast5 files and the called fastq files that were the ou…
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Hey! This issue is basically a revival of #685,
I now have a clean implementation of the Polya-Gamma distribution in https://github.com/theogf/AugmentedGaussianProcesses.jl but I would be happy to mo…
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I thought my error trapping for model structure would allow this but it doesn't seem to. There are easy workarounds, but would be nice if this worked.
```
set.seed(1)
n_sites
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Hi,
I have Pacbio FLNC reads in fastq format. What options should be specified while running the tool. I was thinking
`--data_type pacbio --fl_data`. Is this correct?
Thanks
Abhijit
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Dear Developers of DeePlexiCon,
I just came across this github repo and the paper. I'd love to try using this approach to multiplex with your barcodes. However, I plan to sequence RNA without poly…
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Hi,
We are trying to import 43 studies in the "**crdc/gdc**" folder into our private installation of cbioportal. We got the same error in the validation for every study:
`ERROR: meta_mrna_seq_…
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As the title says, I test --pre-trim-polyx or --post-trim-polyx for my PE read files, but unfortunately, polyA still remains in my R2 file.
The command I tested:
```
adapterremoval3 --pre-trim-poly…