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Hi all,
does shorah algorithms follow the same principle as lofreq?
thanks
Ibseq
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Hi @benjjneb this isn't an issue with dada2 but more a general question. We're using a slightly modified version of the big data workflow implemented in Nextflow for processing PacBio data, but the k…
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Hi,
It's really interesting tool to identify circle DNA from DNAseq data. But, recently, we are considered with the variety of circle DNA length and length of insenrtion of reads, which would influen…
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**Describe the bug**
SARS-CoV-2 variant BA.2.86 frequently has a 12 basepair insertion in Spike protein, which should be called as
MN908947.3 21608 . GTTAA GTCATGCCGCTGTTTAA
Ho…
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Thank you for developing the Cyanoseq database, which is very helpful for aquatic microbial research. I want to use Cyanoseq database on my 16S rRNA gene amplicon sequencing data. I downloaded CyanoSe…
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Hello! I am writing seeking help with primer removal in cutadapt. I have Paired-end sequences, sequenced with Illumina. Illumina's own software removes index and adapters that are not of interest to…
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I am interested in structural variants/copy number variants (SVs/CNVs) of whole genome sequencing data of patients with Epilepsy, but I do not know where to commence with the analysis once the output …
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Hello,
I am trying to download sequences from a set of species with the following code:
crabs db_download --source ncbi --database nucleotide --query 'ITS2[All Fields] AND its2[All Fields] AND …
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Hello all,
Just wanted to let people know what tools I'm working on (or might work on in the future), in case anyone else is interested in these.
I'm concentrating on RNA-seq/ChIP-seq/ATAC-seq wo…
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Hi,
I have multiple 30X WGS data and want to try AA on it. As mentioned in other issues, 30X is too large for AA and downsampling is recommended. But I'm not sure which one is the right way for downs…