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Hi, i am reading your "Unexpected mutations after CRISPR-Cas9 editing in vivo” are most likely pre-
existing sequence variants and not nuclease-induced mutations" paper. In your paper,"in comparing t…
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Hi there,
I am attempting your pipeline with single end reads (since my overlap is over 65bp and I get an error from flash when trying to merge. Is there any way to adjust flash params?). Reads wer…
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- ~~set up directory structure for tries, genomes, and output guides & figures.~~
- ~~script to move genomes & tries from warm storage into compute storage~~
- ~~recover mm10 coding exon region bed …
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* **Download** the audio file from [Soundcloud](https://soundcloud.com/themethodpodcast/010102_08raw).
* **Transcribe** using [oTranscribe](http://otranscribe.com/) and include regular timestamps.
*…
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I'm collaborating with a biologist, and he likes QC plots like those in [Genome-Scale CRISPR-Cas9 Knockout Screening in Human Cells](http://science.sciencemag.org/content/343/6166/84). Figure 2 parts …
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If you include a ComponentDefinition into a ModuleDefinition, libSBOL inlines it into the ModuleDefinition and will not allow it to exist at the top level. This prevents use of a ComponentDefinition …
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We already had this one, but it's not really gone. We currently don't know how to reproduce this one reliably. It is almost certainly data related and happens if an admin gets a list, like a `GET` req…
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I would like to propose a new Production_method tag within the ?Variation model.
MosDEL
```
Origin Production_method MosDEL
```
Thanks,
Mary Ann
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I'm running CRISPRessoPooled in mixed-mode with the following command:
```
CRISPRessoPooled \
--fastq_r1 A5_S184_L001_R1_001.fastq.gz \
--fastq_r2 A5_S184_L001_R2_001.fastq.gz \
--amplico…
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Update the following URL to point to the GitHub repository of
the package you wish to submit to _Bioconductor_
- Repository: https://github.com/OscarBrock/gCrisprTools
Confirm the following by editin…