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Hi guys - when we re-ran everything with the corrected input guide file, it solved the problem of that first guide still being present in the undashed.fasta file. However, we still found that the unda…
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https://dx.doi.org/10.1038/nbt.3300
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[JC04-4-CT13_short.txt](https://github.com/lucapinello/CRISPResso/files/1473019/JC04-4-CT13_short.txt)
[JC04-4-CT13_S4_filter.fastq.gz](https://github.com/lucapinello/CRISPResso/files/1473021/JC0…
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Hi there,
I am attempting your pipeline with single end reads (since my overlap is over 65bp and I get an error from flash when trying to merge. Is there any way to adjust flash params?). Reads wer…
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Hi, i am reading your "Unexpected mutations after CRISPR-Cas9 editing in vivo” are most likely pre-
existing sequence variants and not nuclease-induced mutations" paper. In your paper,"in comparing t…
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- ~~set up directory structure for tries, genomes, and output guides & figures.~~
- ~~script to move genomes & tries from warm storage into compute storage~~
- ~~recover mm10 coding exon region bed …
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* **Download** the audio file from [Soundcloud](https://soundcloud.com/themethodpodcast/010102_08raw).
* **Transcribe** using [oTranscribe](http://otranscribe.com/) and include regular timestamps.
*…
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I'm collaborating with a biologist, and he likes QC plots like those in [Genome-Scale CRISPR-Cas9 Knockout Screening in Human Cells](http://science.sciencemag.org/content/343/6166/84). Figure 2 parts …
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If you include a ComponentDefinition into a ModuleDefinition, libSBOL inlines it into the ModuleDefinition and will not allow it to exist at the top level. This prevents use of a ComponentDefinition …