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The error log file as:
[E::bgzf_uncompress] inflate failed: invalid distance too far back
[E::bgzf_read_block] inflate_block error -1
Number of samples in line and header disagrees at chr2:13176554…
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Hi there,
I am using seqwish to build pangenome graph. I would like to inquire if seqwish supports output with nodes as single characters. Could you please confirm if this functionality is availabl…
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Hi,
I have a design question. My initial reaction to the concept of bactopia-tools and how each subworkflow essentially becomes its own, separate (manual!) command line call was one of "uhm why?!".…
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Hello,
I am a Master Student and currently working on creating a pangenome from 21 longread accessions. A lot of my ideas on how to create my pan gene library was inspired by your work, so thanks in …
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I have a sequence of `27bp length`, and I want to find out the `exact match of this sequence, and all the sequences that have 1 mismatch and 2 mismatch`, by the method of graph.
I used `bowtie1` to…
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Hi
I'm currently trying to load in a BED or GFF file when loading in the GFA. No gene coordinates are overlayed.
I've tried with a BED file and GFF:
GFF:
```
Morex_v3_chr6H pgsb gene 516806…
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Hello,
I've generate a graph genome using the cactus pangenome workflow for five insect assemblies, with small genome size (~230Mb).
The workflow completes successfully, and generates the indexes fo…
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We are trying to align ~5000 bacterial genomes and are finding a number of issues. One of them happens on calling getLongestPath
The problem is due to the default limit on recursion in python, whic…
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Is it possible to use GSAlign to align multiple genomes with each other? Say by concatenating the genomes and using the same file as the reference file and the query file? I know in your paper you rul…
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Hello,
This seems similar to this error [https://github.com/ComparativeGenomicsToolkit/cactus/issues/1525](1525) ,
but I am running cluster where `cactus/2.8.2` is pre-installed. Might the followin…