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It would be good to remind users that the eventDate should be formatted with the ISO format. I suggest at least putting the link to the Darwin Core definition: https://dwc.tdwg.org/terms/#dwc:eventDat…
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Currently DNA term links from Table 3 https://docs.gbif-uat.org/publishing-dna-derived-data/1.0/en/#mapping-metabarcoding-edna-and-barcoding-data go to 15 May version, whereas later one exists https:/…
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Hi,
I ran swarm and clustering with d = 13 (yes, very large, I know. I am trying a few parameters others have used. I am working with animal COI metabarcoding data with high intra-specific variabil…
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Hi Mike! first of all thansk for the tutorial and clear info in your page!
I'm following both DADA2 tutorial (https://benjjneb.github.io/dada2/tutorial.html) and your pipeline too (https://astrobio…
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Been waiting for a package like this. Thanks. Hoping it will be a replacement for my Rstan Code. But I hit walls when running your examples, which is not a great sign.
In the guide, pg 65, example…
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Hi there,
I have .fastq.gz files which have already been trimmed and filtered. I've followed the instructions to import the Silva_132_release database for 16S gene with sequences clustered at 99% ide…
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Hi, I am trying to demultiplex on my metabarcoding data. I found that "sabre" can do it. I have try to install sabre, but face some problem with installing. Hoping for a guide from you all to me to s…
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Specifying ref db:s and algorithms is a good idea, but:
1) the terms _UViGs_ and _vOTU_ in field names _votu_class_appr_, _votu_seq_comp_appr_, _votu_db_ refer to Uncultivated _Virus_ _Genome_ (MIU…
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Wow, this is all really cool stuff! I have basically zero familiarity with metabarcoding, so I just had some questions about it!
First, what's the original source of your samples from the bats/mice…
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Question from @mfisher5. Do we have any Manila clam tissues/DNA she could potentially use?
> I'm venturing into the shellfish world to do some DNA metabarcoding of green / Dungeness crab stomachs,…