-
Hi there,
Here is the whole process I run after dorado 5mC and 5hmC basecalling.
```
dorado aligner reference.fasta dorado_5mC_5hmC.bam > aligned.bam
samtools sort aligned.bam > aligned_sort.bam…
-
### Is your feature related to a problem?
Some reads are inevitably thrown out due to lack of barcode, basecalling errors/below Qscore threshold, etc.
### Describe the solution you'd like
If you ha…
-
# Issue Report
## Please describe the issue:
Does `dorado demux` support the duplex calls. Previously you mentioned this was not implemenetd yet #405?
Thanks
## Steps to reproduce the i…
-
### Operating System
Other Linux (please specify below)
### Other Linux
RedHat 7.9 (Maipo)
### Workflow Version
v2.9.0-gd9e8593
### Workflow Execution
Command line
### EPI2ME Version
_No resp…
-
Hi,
I used Megalodon to call CpG with the following command
```
megalodon $FAST5 \
--output-directory $OUT \
--outputs basecalls mappings mod_mappings mods \
--reference $REF \
--devices $GPU…
-
Hi @dvera , My fast5 file is produced in R7.3 flow cells using genomic sequencing kit SQK-MAP006, how can I set my CONFIG when I use "read_fast5_basecaller.py" for basecalling?
-
If a folder with FAST5 files is provided perform basecalling with guppy and then start the assembly pipleine.
see
https://github.com/replikation/docker_pipelines/blob/master/modules/basecalling.n…
-
We find the reason a read ended to be useful info when looking at direct RNA data. Since some modifications produce higher levels of unblocking events (example: panels K, L, M [here](https://www.ncbi.…
-
Hi everyone,
I was an enthysiastic guppy user and, although i love dorado, i really miss the barcoding_summary.txt file that was produced during demultiplexing/barcode-trimming (and, as far as i've…
-
# Issue Report
## Please describe the issue:
Hi,
we just saw that Dorado v0.7.1 is out and there are fixes for autobatch calculation with modbase models when trying to call on multiple GPUs.…