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Salivary microbiome profiles for different clinical phenotypes of pituitary adenomas by single-molecular long-read sequencing – Xuefei Ji – Microbiology Spectrum
https://journals.asm.org/doi/10.1128…
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Hello, is this tool suitable for long read sequencing ?
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Dear Kristoffer,
Thanks for developing and maintaining this tool.
I just want to ask a quick question on what to set as "readLength" on the importIsoformExpression function for Stringtie outputs f…
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fastq-to-ubam naming convention for output:
```
/${sample}/HAS_READ_GROUP/{readGroup}/HAS_FASTQ/${jobRequestId}_${sample}_${readGroup}.ubam
```
1000 Genomes Fastq name:
```
ERR3239279_1.fastq.gz
``…
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When calculating depth by GC content, samtools stats command ignores reads overlapping boundary between two GC bins. For short read data, it doesn't matter since, as explained in [stats.c](https://git…
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Hi,
First off, great tool! This is exactly what I was looking for.
I have two questions, the first is regarding how to run flexiplex when your barcode and UMI are on different sides.
My read s…
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### Description of feature
Due to the high fragmentation of ancient DNA data, there is not much gain in using long-read DNA sequencing methods for assembling ancient DNA samples. However, many ancien…
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Hi, I have a question about how reads are assigned as conflicting, unassignable etc.
I've been trying to use SNPsplit on noisy PacBio long reads to help with haplotype-resolved assembly (which I kn…
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We realised that the current damage model used in `--singleStrandMode` is not appropriate if the input pileup data contains alleles from unmerged R2 reads from paired-end sequencing. Currently, this m…
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Good afternoon,
In the config file for `MKFQ`, there is a final parameters `Barcode_Length`. In the example files provided by google drive, the barcode length is `54` for the 10x example. I have so…