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When running the commands under the section "Prediction" at http://kplogo.wi.mit.edu/manual.html#examples, I get a segmentation fault after the second command.
I've tried this on CentOS 6 and from …
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Hi I am using crispr guided to a target region. we then sequenced the genomic dna (pcr product) that targets this region using miseq 2x250 + 10bp Index1 + 8bp Index2 (as defined in my previous questio…
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For GeCKO V2 libraries, some gRNAs hit multiple genes by design (i.e. they have different gRNA ID but same DNA sequence). Currently, only one gene symbol per DNA sequence is shown. e.g.
```
>POTEE…
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Dear all,
currently we work on increasing the speed of the FASTQ data extraction and mapping.
We expect a speed increase by 4-9x compared to the current implementation.
I will close this once it …
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Some parts of the [code example for constructing a CRISPR circuit PDF](http://sbolstandard.org/wp-content/uploads/2016/09/untitled-1.pdf) are unclear or don't cover steps that the user encounters in E…
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The vignette recommends the usage of Bowtie 2 to map gRNAs to the library sequences. However, there are a number of issues on Bowtie 2's [tracker](https://github.com/BenLangmead/bowtie2/issues) highli…
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On line 168 and 169, the two sequence constraints should be created for "gRNA_b_gene", not for "cas9m_BFP_gene".
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Deepak: Could you please look into it in case there is any problem.
20150626 email:
Ye Chu (ye.chu.test@gmail.com) sent a message using the contact form at
http://www.peanutbase.org/contact.
I was t…
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In oder to distinguish the interactor that have a
sequence (nucleic acid and protein) from those who do
not (small molecules, complexes) we propose to add the
term "polymer" in the PSI interactor type…
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In oder to distinguish the interactor that have a
sequence (nucleic acid and protein) from those who do
not (small molecules, complexes) we propose to add the
term "polymer" in the PSI interactor type…