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Hi:
I've finished a modified base model.And I found strange result: `basecalls.fa` which is generated by basecall.py with final check model are quite different from `modbase_references.fasta` which i…
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Hi:
I I understand processing RNA data requires a transcriptome due to the lack of spliced mapping support within the Tombo.
However, I used the genome for re-squiggle and it also achieved goo…
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What sort of fast5 files is expected as an input for the
`python2 fast5ToEventTbl.py input.fast5 > output.event.tbl`
step?
I was trying to use both single and multi read fast5 files, generated fro…
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Update the following URL to point to the GitHub repository of
the package you wish to submit to _Bioconductor_
- Repository: https://github.com/dtm2451/dittoSeq
Confirm the following by editing…
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#### Scientific goals
In https://github.com/AlexsLemonade/OpenPBTA-analysis/issues/73 it's noted that the reported sex of the participants in the study didn't align with information from germli…
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Hello,
I'm doing RNA-Seq with ONT MinION (direct cDNA sequencing). After I align my reads to the reference genome via minimap2, I want to look at QC plots with NanoPlot. It always crashes because `…
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all reads get "Alignment not produced"
i'm having trouble to understand whether the problem is in the reference file (i'm sequencing direct-RNA samples and used 'GRCh38_latest_rna.fna'), or in the ma…
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Hi Adnan, great software, congrats!
Quick question: tail_start and tail_end numbers are in coordinates of the "samples" (signal/raw data), is there a way to report it in terms of the basecalled rea…
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How much coverage do we need for a direct RNA sequencing experiment that would be optimum for identification of m6a sites ?
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Hi:
When I execute the` tomb text_output browser_files` command on the output file of `level_sample_compare`. I got two files.
The first question is that I compare two sets of direct RNA sequencin…