-
Hi Haoyu,
I get this assertion failure with both 0.19.4 and 0.19.0:
hifiasm: gfa_ut.cpp:4917: void uc_block_qse_cutoff(uint32_t, uint32_t, uc_block_t*, uint32_t*, uint32_t*): Assertion `(*nqs) >= x…
-
Hello,
I tried to replicate the Zymo D6331 assembly described in the mDBG manuscript. The assembly and the graph simplification finished successfully, but sequences were all placeholders. Somehow I…
-
A reviewer for our last paper suggested to compute the LMM Kinship matrix from unitigs presence/absence patterns, so that core/accessory population structure is fully account for. Could this be useful…
-
Hi,
While i run the assemble.sh I am getting the following error
[Tue Feb 5 18:16:54 UTC 2019] Processing pe library reads
[Tue Feb 5 18:19:19 UTC 2019] Processing sj library reads
Invalid…
-
Hello,
I am trying to assemble a viral genome (~ 190 kb). But, it has delivered 0 contig when I set '-g 190k' with a questionable warning message ("** WARNNING: input file is not in gzip format **…
-
Hello,
After performing a unitigs analysis, i put the generated plot and the .gff file in phandango and i have something like this:
![image](https://user-images.githubusercontent.com/74925521/1378…
-
Hi,
I am looking to find Unitigs from the antimicrobial resistance genes generated from ResFinder. Please let me know if the pipeline works to predict Unitigs from the files containing antimicrobial …
-
Hello again!
I've noticed a major slow down in annotation speed for a graph I'm annotating and I was wondering it's to be expected or something you'd like to look at.
The speed difference can be…
-
Thank you for really amazing tool.
I have faced with some errors when I tried to use Ratatosk. Generally it was a time or memory limit errors. Execution have been stopped on the indexing stage 1, a…
-
Dear author
I am annotating a set of significant unitigs following the manual: https://pyseer.readthedocs.io/en/master/usage.html#annotating-k-mers.
When I run
`annotate_hits_pyseer signific…