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Hi,
I used Canu in the past and worked great! We recently did a migration to a new server (SGE) and I have been trying to run a job there, but it keeps stopping before the last step, failing to ali…
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### Subject of the issue
Hello Pierre ,
I want to use SamFixCigar on mapping data from MinION R9.4 datatsets (https://github.com/nanopore-wgs-consortium/NA12878), to get .sam files with a CIGAR no…
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I am testing using Canu with 25 Nanopore 1D reads. I am getting failure notices but I am not sure how to fix the error. Here is the code I ran:
```
#!/bin/bash
#PBS -l nodes=n03:ppn=8
#PBS -l …
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Hello Canu team,
I'm trying to run canu to assemble a 14 MB genome with MinIon reads.
It works find for a couple of samples, but in two of them I get this:
```
-- Canu release v1.5
-- Detect…
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Hi.
I'm using the data from [NA12878 Human Reference on Oxford Nanopore MinION](https://github.com/nanopore-wgs-consortium/NA12878)
and I found that the replayfast5.py demo didn't work well.
The …
ban-m updated
7 years ago
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Hi,
I am having an issue with canu. I used Homebrew to install on my Mac. (10.12.5) 128 GB memory, 4 TB HD space. I had a 4 Core processor in the machine and canu would work, but would take a whil…
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Hi,
I used picopore successfully to a) do lossless compression on 0.5TB input and b) further do deep-lossless compression on the results of a).
Now I am trying to get rid of all metrichor baseca…
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Hey Wouter,
Awesome set of tools you've made. Many thanks!
I work with a vertebrate genome and need to run multiple flow cells using the same input tissue/DNA source. Imagine I've completed a tr…
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Hello
I am hitting an issue with canu failing at:
cd unitigging/4-unitigger
./alignGFA.sh
GFA alignments failed.
I am trying to assemble the lamda sequence reads from the ONP la…
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Hi,
For my master studies I have to work with raw minION data. However, in the fast5 files I can not find the raw data due to the fact that this data is already basecalled. Since you work with raw…