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Clone Manual Dilution steps from RNA workflow in Seq Pooling (SC does not do any library pooling, however).
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Hi,
I did use MACA for single-cell RNA-seq annotation successfully some time ago, however since the OS reinstallation of my machine it stopped working.
My workflow in python 3.10.4 looks like th…
Edert updated
2 years ago
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I have some suggestions for documentation related to analyzing visium datasets posted here (https://data2intelligence.github.io/SpaCE/articles/visium_BC.html).
1) In SpaCE analysis, If a gene exhib…
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Hello,
Thanks for creating this wonderful tool. The time you have put into documentation is really admirable.
I noted that the tutorials are split up in some cases by the data modality (convent…
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Hi, scMAGIC team
I was also playing this package with Seurat object, an error also occoured:
```
> seurat.query table(rownames(seurat.query) %in% rownames(seurat.ref))
FALSE TRUE
20535 …
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adata = sc.read_10x_mtx(input_file)
sc.pp.highly_variable_genes(adata)
-------------------------------------------------------------------------
Output:
ValueError: Bin edges must be unique: arr…
jhu99 updated
2 years ago
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#1 What are the changes required if using sc-RNA seq count data simulated using popular methods like splatter compared to real data?
#2 Methods to integrate different sc-RNA seq data sets?
#3 Change…
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The auto_detect_tissue_type function explicitly uses "pbmc" object. I propose the below correction to fix this issue.
We now pass seuratObject instead of the matrix.
Best
```
# GNU General …
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Hello,
I find dynamo a great tool for single-cell analysis tool.
However, I found it hard to install this package because the network of my cluster is bad.
Is there possible provide a docker image …
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Hi Dr.Qiu,
I am recently analyzing scEU-seq data and conventional scRNA-seq data with dynamo, where I have found a huge batch effect between samples of different developmental stages or different l…