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This is from Marty on our 10X protocol:
> Experimental procedures followed established techniques using the Chromium Single Cell 5’ Library & Gel Bead Kit (10x Genomics; https://assets.ctfassets.ne…
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Sorting by `projectDescription` fails to properly sort all results.
```
$ http 'https://service.azul.data.humancellatlas.org/index/projects?size=30&catalog=dcp37&order=asc&sort=projectDescription&…
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Running TRUST4 on 3' 10X genomics RNA-seq results in B cell inference where no B cells are present, any advice on fixing this issue?
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Hello,
Thank you very much for this vision.
Would you please direct me to the code used for the systematic KO analysis. I generated a gene_barcode expression matrix downloaded from 10x genomics and …
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Thank you for the good tool . I have a question to ask you for help, when using Kraken2Uniq to mapping , which reads should be choose for 10x genomics data? Both or only read2? The read1 only contain…
oyxf updated
3 months ago
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Good afternoon,
In the config file for `MKFQ`, there is a final parameters `Barcode_Length`. In the example files provided by google drive, the barcode length is `54` for the 10x example. I have so…
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Project: ENCODE
So - libraries for next-generation sequencing used to all be made by hand. However, we are starting to see the emergence of platforms (machines) which do the library construction i…
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Hi,
I have single cell BCR sequencing from 10x genomics that I analyzed with the latest version of mixcr and its dedicated preset 10x-vdj-bcr.
I would like to explore the results with immunarch bu…
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Hello,
Thank you for making this tool! I have been struggling with supernova assembly from 10x data due to big genome size (4Gb). Does physlr have any requirements in terms of computer resources f…
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Hi there!
Could you please instruct me in importing data generated by 10X genomics CellRanger to SINCERA?
I'm really eager to use the TF prediction function in SINCERA.
Thank you so much!