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Hello,
This is similar to [issue#69](https://github.com/chhylp123/hifiasm/issues/69) but for me it is unsolved yet. I am running hifiasm v 0.14.2-r315 to assemble ~3.5Gb genome. My hifi fastq files…
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Hi, thanks for your pipeline, I installed the pipeline followed the instructions, the error comes out as missing files, I believe it's not take input in the demultiplex stage, and I don't know why the…
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Hello sir,
Long time fan and user of Flye, Abruijn, etc.
I have three datasets:
- PacBio HiFi
- 90-95% accurate ultra-long ONT
- Hi-C
I know there are other assemblers that may out-perform…
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I am very grateful for you creating such a useful tool.
I am currently trying to use the data from your study for my research and encountered some difficulties. Specifically, I am unsure whether the …
HY6Wu updated
2 months ago
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Hi,
I have used hifiasm successfully several times in the past. However lately I am running into some strange behaviour.
The subject I am trying to assemble is half the genome size of Californian …
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Hi there!
I've been battling with an issue that I'm sure it has already been addressed at some point in other posts on this page. However I would like to understand why my analyses are failing the …
ddols updated
3 weeks ago
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I am running `hifiasm` of ~1.4GB genome using both the ONT and PacBio HiFi data. The program is stuck at `[M::ha_pt_gen::21255.245*7.18] ==> indexed 5753798835 positions, counted 251767750 distinct mi…
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Dear autor：
I am assembling a plant genome, and now it is assembled to the level without gap. It have 21 chromosomes, but so far only 39 have been counted, and there are 3 motifs (AAACCCT) w…
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I get a segmentation fault when I try to run the RAFT stage using the following command:
```
COVERAGE=$(grep "homozygous" "${OUTPUT_PATH}/1_HIFIASM_ASSEMBLIES/${SAMPLE_NAME}/${SAMPLE_NAME}.errorco…
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Hi,
We are looking to optimize the polishing step for reconstructing a plant genome using PacBio HiFi reads (Q30). Specifically, we tested both NextPolish and NextPolish2, followed by a variant cal…