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I have a genome de novo and hybrid assembled (~2 Gb) using ONT and Illumina reads and Masurca, Flye, and Shasta assemblers. Each assembly is now being error corrected/polished via Racon and then Pilo…
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an issue report by @lexnederbragt in email using `falcon` for hybrid reads
Than I saw something. I ran this command
```
/node/work/lex/falcon/fc_env/bin/fc_run.py fc_run.cfg
```
and save stdin and…
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[string_serialization](https://linkml.io/linkml-model/latest/docs/string_serialization/) isn't actionable like `range` or `pattern`. It's intended as a hint for how a software agent might want to popu…
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Hi Paul,
I have run framefix on a few bacterial hybrid assemblies (ONT+Illumina) generated with Unicycler or Flye/Racon/Pilon. I am using the Uniref90 database:
/path/to/framefix /path/to/assem…
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I have assembled a bacterial genome from Nanopore long reads using Flye and would like to use polca to polish them using the Illumina paired short reads. I have installed Masurca by cloning from githu…
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For the hybrid assembly track, the goal is to generate a complete assembly where all the contigs have the tag `circular=true` (given that this is biologically true). However, this is not always so eas…
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## Steps to Reproduce
1. AOT 4.5, GAC and mono/msbuild/15.0/bin with `mono --aot=hybrid --debug`
2. Do an msbuild invocation on a solution like MD's Main.sln
3. runtime crashes
4. The problem is…
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Hi all,
I usually run `unicycler` on minion + illumina reads but got some raw reads from pacbio the other day and wanted to try it. I used `bam2fastq` to get fastq files from the pacbio bam files, …
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Hello @chhylp123
I used hifiasm in trio binning mode using Illumina reads from the parents and HiFi reads from the hybrid. The resulting assemblies (hap1 and hap2) have high duplication levels and…
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Hi,
I have ONT an Illumina reads from a NCTC _Staphylococcus aureus_ strain. I've been able to:
- Perform Canu assembly with ONT reads
- Perform hybrid unicycler assembly with ONT+Illumina read…