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貼吧活動:(請查閱 [SARS-CoV-2 Timeline by 2020.02.21](https://github.com/agorahub/_meta/blob/agoran/theagora/sari/Memorandum_2020-02-21_SARS-CoV-2-Timeline_Nathan.pdf?raw=true), by Nathan :cloud: )
- Colla…
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As we make progress with processing and interpreting CRISPR results from
Brain CRISPR #2821
BIOGRID #2920
we need to design a public CRISPR widget for the 23.06 release - keeping in mind that …
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Dear developers,
Thank you for providing this useful and effecient tools for sgRNA design !
It seems that the guidescan2 could output paired-guide sgRNAs for target genes according to the fig 1 in…
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In my checks, I see:
```
> ## Quick example without off-target searching or annotation
> ## First generate data with the sgRNA_Design Function
> testseq usergenome gtfname alldata
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Hi Mudra,
I'm just wondering if you know what this error is. I simply just `git clone` your repo and ran
`python base_editing_guide_designs.py --input-file ./Sample_data/GFP.fasta --variant-file …
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Hi! I was pointed towards here through the ENCODE preprint ["Multi-center integrated analysis of non-coding CRISPR screens"](https://www.biorxiv.org/content/10.1101/2022.12.21.520137v1.supplementary-m…
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Hi, I was wondering if I could use my interested sequence as input for the sgRNA design (cas9), on/off target score? If possible, is any length limitation for my input? Thanks
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The input bcf files for the tutorial for "[Design sgRNAs for allele specific excision of the gene MFN2 in the WTC genome](https://github.com/keoughkath/AlleleAnalyzer/wiki/Tutorial:-Design-sgRNAs-for-…
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Hi Vincent, when I run the rscript sgRNA_library_design_cmd.R, I always got the following error information:
....
2022-06-25 23:55:31: Scoring sgRNA - binding site combinations...
2022-06-25 23:55…
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Thanks for this great work.
We are looking to replicate the portion of your computational pipeline enclosed by the red rectangle (below).
![mimosca](https://user-images.githubusercontent.com/333…