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I get the following error while running a standard alignment .
Error [M::bwa_idx_load_from_disk] read 0 ALT contigs
It will be great if I can get some help to see what's wrong. I can send the raw …
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Hi! SNPsplit seems like a perfect tool for allele specific research, nice work!
Here is my question:
I hope to look at RNA expression at individual chromosome, but although my reads are processed…
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## Preferred term label
methyl-c sequencing assay
## Synonyms
mC-seq2
## Textual definition
A sequencing assay (library preparation method) that involves fragmentation of genomic DNA fo…
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I'm not sure whether this is a desired behaviour or not, but I've noticed that calling qAlign as follows on aligned data results on a QuasRprj object which doesn't retain the aligner information
`p…
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Where can I find the explanation of each flag in the SAM output of Bismark, such as ZC, ZR, XL, XA, XB and YD?
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Hello,
I am running yap demultiplex with YAP with the following command:
yap demultiplex --fastq_pattern "/path/to/fastq_files/snmC-seq2/*/*fastq.gz" --output_dir /outdir/demux_output --config_p…
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Dear @smarco
I think this question ( https://github.com/smarco/gem3-mapper/issues/21) should be related to gem-mapper, I just want to know how to set the `--underconversion-sequence`, `--overconv…
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Hi!! I am struggling a bit with the alignment of my 150 paired-end read PBAT libraries. I removed any adapters and trimmed 9 basepairs from the 5' and 3' end of both reads using TrimGalore v.0.6.6 as …
mo716 updated
3 years ago
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Hi Felix,
I'm having an issue with the SNPsplit_genome_preparation (v0.5.0) processing step. There seems to be an issue with variable definition, but I'm unfortunately not familiar enough with Perl…
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https://mp.weixin.qq.com/s/qqYzrXlM8CDCC-CEoZj-_g
ixxmu updated
3 years ago