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I have a bam file which was basecalled by Dorado, and I try to run deepmod2 on it using the latest model bilstm_r10.4.1_5khz_v4.3. However the Dorado basecalling used for that bam file is v5.0 rather …
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Hi
I have a FASTQ file generated with MinIon Minknow and the fastq headers contains following model information `2021-05-17_dna_r9.4.1_minion_96_29d8704b`. I am using Clair3 for basecalling and I h…
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Hi
It is great to hear the support for long-reads coming to kallisto I would like to introduce it to our pipelines
I have few question regarding running lr-kallisto on ONT dorado basecalled rea…
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ONT has transitioned to a new basecaller that can now, by default, call poly A tails.
Is it possible for ninetails to work with these new formats? I know the current workaround for tailfindr is to…
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Dear @all
I think this question would be more appropriate here, so I referenced it here (https://github.com/nanoporetech/dorado/issues/1089)
Thank you for your time.
Kind regards
Zheng zhuqi…
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Hi
I am using herro since last weekend when ONT included this into their dorado 0.7 basecaller (i am running through dorado). I have 10.4.1 ONT data of bees (some of them are male, i.e. haploid)
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Hey @lh3
We are starting to add alignment support to [dorado](https://github.com/nanoporetech/dorado) and I would like to get some patches upstreamed if possible.
The following pull requests ht…
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Hi! From what I understand, Remora is integrated with Dorado, and the latest SUP canonical basecalling models now use Transformer-based architectures. I was curious if the SUP models for modification …
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Hi
After an issue with our MinION Mk1C we recovered non-basecalled files into pod5 files - using the recommended `/opt/ont/minknow/bin/recover_reads` command of MinKNOW on the device.
However, …
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Hi everyone,
I ran some samples using an updated Dorado model and I'm getting different thresholds from Modkit for the same sample. In my case, I have a lower calling threshold compared to, for examp…