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https://mp.weixin.qq.com/s/Fhaj6_gtqGRMEbpUsySnFQ
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I used MSConverter to convert Agilent .d file to .mzXML file. Then use Fragpipe for database searching.
The Run was stopped. Please help!
System OS: Windows 10, Architecture: AMD64
Java Info:…
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Hello again @arunhpatil ,
I'm currently working on modifying miRge3.0s alignment behaviour for my own purposes and I've noticed a routine in the code which may have the potential to output non-opti…
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NTR piRNA-mediated transposon silencing
NTR tRNA-mediated transposon silencing
https://pubmed.ncbi.nlm.nih.gov/33789107/
Split from https://github.com/geneontology/go-ontology/issues/231…
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I want to create a new human reference that includes some custom synthetic miRNAs. It seems like I can't add a new set of miRNAs to an already existing reference, so I have to create a new reference f…
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Thanks for your replies to my previous posts @almeidasilvaf! Your detailed responses are much appreciated. I've got another puzzle for you though.
I found something strange in the `.collinearity` f…
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I started to tag some ncRNAs with
/db_xref="SO:0002247" (small non-coding RNA)
Do we automatically recognise these in Chado, or does it need adding to a mini ontology
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Hi,
After running TESmall on mouse small RNA samples, I get a counts summary file that has a lot of repeated entries specially for the tRNAs, this will hamper proper DESeq2 functioning. Is there a wa…
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I installed Mirge3.0 using conda and running using the following line obtained from `run.log`:
> /home/ubuntu/anaconda3/envs/mirge3/bin/miRge3.0 -s 1.fastq.gz,2.fastq.gz,3.fastq.gz -lib /home/ubunt…
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**Is the bug primarily related to salmon (bulk mode) or alevin (single-cell mode)?**
Salmon
**Describe the bug**
Only `u` (unmapped) reads are labelled in the `aux_info/unmapped.txt` file, even w…