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GoldRush is a de novo genome assembly algorithm with linear time complexity in the number of input long sequencing reads.
Repository: https://github.com/bcgsc/goldrush
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Hello, I want to add your data to my studies.
Could you provide the expression matrixs from your paper:
`High-throughput targeted long-read single cell sequencing reveals the clonal and transcriptio…
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I was curious if you thought Racon would suitable as a long read polisher. E.g. if one had a completed assembly which is 99.9+% correct, could Racon use long read alignments to help fix up the remaini…
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**Project:**
10x Genomics (http://software.10xgenomics.com/) has developed a technology that produces new type of long-range sequencing data called Linked-Reads. Long (>100Kb) double stranded DNA is s…
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Hello,
Our lab has been using hifiasm for a number of genome assembly projects. We are using Nanopore reads (kit 14 chemistry, R10.4.1 flow cells) that have been error-corrected with PECAT or HERRO…
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$ python reago.py filtered.fasta out -l 150
...
Traceback (most recent call last):
File "reago.py",line 823 , in
correct_sequencing_error(subgraph,5)
File "…
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Hi, thank you very much for sharing this tool.
I would like to know two questions:
1. what is the difference between cutadapt and porechop, which is another adapter trimming tool.
2. How about …
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May I ask if FINDER is compatible with long read sequencing? If not, how do you recommend enhancing the annotation results with the FINDER output using such data?
Thanks a lot!
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Hello Vamb team,
With the same parameters for short reads binning, I used it also for a PacBio long reads sequencing project. It turns out that nearly each long contig was considered a bin (several…
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Dear developers,
We have developed our own Nanopore protocol which involves custom barcode sequences. They are longer and differ from the Oxford Nanopore official barcodes.
We are starting to use Do…