-
Hi Kristoffer,
I understand that each cluster generated by isONclust represents all reads that came from the same gene.
However, I was looking for a de novo tool/method that could determine wh…
-
Hey Leandro!
I have been taking a look at cases where Pandora incorrectly annotates on gene as another. I took the first allele in each MSA that build my panRG and used Pandora map adding the `--d…
-
Hello, I'm trying to assemble some reads with minimap and miniasm. Minimap seems to have worked, but my process is failing on miniasm:
```
[M::mm_idx_gen::0.010*2.97] collected minimizers
[M::mm_…
-
Dear Kraken2 developers,
thanks a lot for this fantastic software! I really appreciated adding the --report-minimizer-data flag (borrowed from KrakenUniq) to latest versions of Kraken2, because bre…
-
This is an issue related to vgteam/vg#3328.
We have assemblies for two genomes and I tried to build a graph with pggb for one of the chromosomes (about 1.8 Gb in length, mostly repeats). I have a 1T…
-
I found that Minimap2 sometimes generates an unusual order of CIGAR opertations; with some `N`s, followed by some `I`s then followed by more `N`s instead of the more expected order of having all the `…
-
Hi @Maggi-Chen, I'm use the test supplied on the github page and I've run into the below errors
```
(fusions) ryleyd@minion:/data2/ryleyd/FusionSeeker$ ./fusionseeker --bam testdata/test.bam -o t…
-
In the document about PPtr, it is mentioned that the target object should be allocated through heap.
However, in the low-level programming, many objects could be allocated without using heap.
I…
-
**Copy and paste the exact command you tried to run**
### First I tried running minimap2, followed by flair correct:
```
module load minimap/2.28-IGB-gcc-8.2.0
module load SAMtools/1.17-IGB-gc…
-
Hello.
Is there any limit to the length of chromosomes in chromap?
I'm working on an contact map for genomes with chromosomes greater than 2GB, and some other alignment tools seem to have length…