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Project: [PSMFC-mytilus-byssus-pilot](https://github.com/mattgeorgephd/PSMFC-mytilus-byssus-pilot)
Background: We have tag-seq data from the gill and foot (feet?) of mussels (_Mytilus trossulus_) e…
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**Operating system**
Linux
**Conda environment**
```
_libgcc_mutex 0.1 main
_openmp_mutex 5.1 1_gnu
avro-python3 …
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Hi ,
I took the iso-seq at BGI.
I used the official PacBio pipeline flnc.fasta file which has only a hundred sequences, which is obviously not correct.
I used your pipeline and found that the numbe…
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Hi, thanks for developing SQANTI3! I have a question maybe it is referred to #103
I used SQANTI3 to analyze Pacbio Iso-seq data. I found that the values of ORF_length are not the same as the real …
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Hello,
Thanks for creating TAMA and also creating a great wiki to understand the I/O of TAMA. It helps so much.
I have 4 long read samples, that I processed via Pigeon and got classifications (…
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Hey there, Rajewsky lab!
I downloaded samples SRR17321895 to SRR17321901 (7 total) from the SRA.
I wanted to preprocess them according to the recommended way in the isoseq3 guide, as instructe…
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Hi, I'm looking to see if I could use the QVZ lossy compressor in SPRING for PacBio HiFi reads. I'm currently testing on the following FASTQ file (https://github.com/PacificBiosciences/DevNet/wiki/Seq…
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Dear Pacbio developers
I am analyzing MAS-Iso-seq data for the first time, and comparing it to the 10x short read results.
I would like to calculate the UMI dedup rate to make sure the sequencin…
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Hi,
Hifiasm is a great piece of software.
I get two haplotypes with HiFi reads and HiC reads. This is a wild diploid plant, so I don't have parental information.
I want to know how to evaluate …
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Hi,
I found that there are many incomplete gene models in mikado output gff file, is there a way to update these models to complete gene models?
Best,
Kun