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**1. What were you trying to do?**
VG stats on 3 files after aligning them using vg giraffe and the T2T-CHM13 reference.
**2. What did you want to happen?**
vg giraffe to use paired-end mappi…
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```
I have been using STAR with Illumina 101bp paired end reads. The first set of
libraries I sequenced work great going through the pipeline, but I have had a
very strange problem with the most rec…
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Version 0.23.4
I get a different number of reads and bases after filtering every time I run fastp
```
(base) exouser@julin-2:$ for i in $(seq 1 10); do echo "run $i"; fastp --in1 GH.lane67.fas…
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Hi Zhigui,
Thanks for providing this nice workflow. Now I have a longread hifireads for genome assembly and I have finished step1 and step2.
The step3
bwa mem -M -R "@RG\tID:${s}\tSM:${s}\tPL:I…
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Hi there, I`m using woltka classify on metagenomic short reads. I got the output as RPK which gave me the copy number of each gene in each sample. Although, I`d like to normalize the sequencing depth …
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Hi,
I am running paleomix bam_pipeline, I want to use bwa aln when the reads shorter than 70bp, and when read longer than 70bp, using bwa mem algorithm, I want to set this, could you share your h…
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I am using deeptools bamCoverage to analyze short (35-80bp) paired end fragments. All reads in my bam file are properly paired based on samtools flagstat and Picard. Yet bamCoverage extends some a…
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**Is your feature request related to a problem? Please describe.**
I deployed alluxio version 2.9.0 (alluxio/alluxio-dev:2.9.0) with fluid 0.9.1.
Then I used 2 nodes A and B, here is my vdbench tes…
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Dear njaupan,
I use ecc_finder to assembly ECCDNA from two paired short reads,but the Unicycler.log said it was faided and the spades_assembly directory was deleted. How can I set options to get a co…
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Hi,
I have tried polishing an assembly with short-reads (HiC reads with -unpaired option in cfg) and long-reads (nextdenovo error corrected reads). But is taking longer than expected (before 5 iter…