-
I'm curious if anyone knows how to appropriately merge multiple single cell datasets to create a reference dataset that's compatible with MuSiC? I'm trying to deconvolute a bulk RNA-seq dataset from b…
-
Dear developer,
Many thanks for the great tool! It will be very impactful for the community as shown by its application to the Zanzibar dataset.
Is it possible to adapt the tool to estimate relate…
-
Hi @alexdobin
Thanks again for writing & maintaining STAR. The latest diploid mapping feature is very cool. I was wondering how difficult you think it would be to extend it even further, to map to …
-
Hi,
I have data obtained from two different single-cell RNA-seq platforms:
* **icell8**: **specific cell type** obtained by flow cytometry
* **10x**: **all cell types except the above**
Now,…
-
Hello Sir/Ma'am,
A very convincing normalisation approach well explained in the paper.
So we have the allele specific read count from Single-cell RNA seq data without a…
-
Hi,
Thanks for developing this really interesting methodology. Would it possible to give us a short tutorial on how to use one's own single cell RNA seq data for protein prediction?
Current demos …
-
`computeCommunProb` fails with aggregate error.
```r
> cellChatData
An object of class CellChat created from a single dataset
21473 genes.
101722 cells.
CellChat analysis of single cell RN…
-
Dear Author,
I am using this script for analysis:
import SEVtras
SEVtras.sEV_recognizer(input_path='/home/yeziyang/Sc', sample_file='/home/yeziyang/Sc/Sc1_LN', out_path='/home/yeziyang/Sc/outputs…
-
Hi
I need your personal point of view please
I have two `bulk RNA-seq` patients (PBMC) on which I run `mixcr`
For the same two patients I have `5' single cell` on which I run `TRUST4`
I a…
-
Hello,
Thank you so much for such a useful tool. I have been applying this to single-cell multiome (RNA-Seq plus ATAC-Seq) libraries, using the parameters as recommended here for the different libr…