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Hi,
Dorado Developers.
Firstly, I'd like to express my gratitude for developing such an innovative tool for the Nanopore sequencing community. The addition of the "--estimate-poly-a" parameter in D…
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Hi all,
The read_clustering step failed for whole operons (16S-ITS-23S, ~4.1k mean read length).
The whole nextflow pipeline ran through without errors (on a Mac M1) with test fastq files `mock4…
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The whole production system is a bit chaotic and old. It uses eval which I also don't like at all.
- [ ] Refactor resources setting.-
- [ ] Refactor infos.-
- [ ] Refactor the planets resources upd…
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I'm trying to run the read simulator in the perfect mode with the following code:
`simulator.py genome -rg simulated/simulated.fasta -k 6 -b guppy -s 1 --perfect --fastq`
But it generates an err…
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Dorado wont install completely and gives the following errors:
```
/home/datavis/dorado/dorado/splitter/myers.cpp: In function ‘std::vector dorado::splitter::myers_align(std::string_view, std::strin…
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Hi,
I'm using the demux tool in Dorado, and I am very happy about the feature to translate barcode IDs to user inputted sample names (aliases) from a sample sheet. However, you have set a max limit…
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### Operating System
Other Linux (please specify below)
### Other Linux
Ubuntu 20.04
### Workflow Version
wf-amplicon v1.1.1
### Workflow Execution
EPI2ME Desktop (Local)
### Other workflow ex…
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I am obviously doing something wrong, but I don't see the problem :-)
Running a P2 flowcell with a pool "SAMPLE_ID" containing four libs/barcodes (barcode01-04).
Basecalling and demultiplexing i…
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Hi everyone,
I have a question regarding the analysis of data derived from modkits. I am working with two sets of data (for the moment, a few samples): one concerning samples sequenced without PCR am…
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Hello,
I saw recently in a post that future versions of DNAscent might output modBAM files instead of .detect. I wanted to ask a few questions so that I can anticipate what I need to do for some to…