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Hello,
I'm using CuteSV on Nanopore data with 30X coverage. My sample has a known duplication that isn't making it into the final output but it is detected in the signatures file. I was wondering i…
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Hi, This is not an issue but a question.
This looks like a very interesting and useful tool for what I need. I am however not sure if I am failing to understand but can the tool be used on finished…
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Hi,
I was wondering if there might be a recommendation on using herro corrected reads with assembly mode 2, as opposed to just blindly using config `Nanopore-Phased-R10-Fast-Nov2022` and `--Kmers.k …
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Hi @rjchallis @epaule,
I have problem with loading mapping data and vizualization in bloobtools. My command was - ./blobtools2/blobtools add --cov ~/Desktop/bioinformatics/K17_4_S9_L1L2_R1.fastq.gz…
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Hi, @hasindu2008
When I use f5c to eventalign RNA004 data, the system popped up this bug and crashed.
Here are a few screenshots.
Here is my code:
`for i in input; do`
`echo $i`
`mkdir -p…
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Hi, I am trying to align 16S/ITS read from nanopore sequencing against 16S/ITS reference. bwa-mem2 (v2.0pre2) do well in building index. But it fails in the alignment step.
bwa-mem2 version: 2.0pre2…
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Hi,
I use the freebayes to call SNPs and InDels in polypoidy genotype from HiFi aligned bam (120x for haploid genome). And also have the Ultra-long reads aligned bam (reads N50 50kb) 60x. But the b…
baozg updated
3 years ago
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I'm running nucdiff on a uge cluster, attempting to use 10 processor. The process starts and appears to go well but I never get any output. I'm including my command line and the output.
If you can …
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Hi,
First of all, great job developing IsoQuant, it's a very useful piece of software.
I want to confirm: is the read_group: file functionality currently working as intended in the release vers…
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Hi all,
I would like to use both long read (isoseq) data and paired-end RNA-seq (Illumina) data to produce gene assemblies. what would be the recommended way to do this? I am thinking of doing someth…