-
hi i had bam files unsorted i created with STAR. then i used the Quantify IR bam mode (on unsorted files) with reference genome i buit with irfinder
then i tried to do the Generalized Linear Model w…
-
**Are you using the latest release?**
1.8.13
Hi there,
I am using funannotate to convert a gff3 to a .tbl file, then to convert the .tbl file to a .gbk file, and then to spit out the transcript…
-
In the third step, InPACT_quantify is running with the following error, and I would like to know which file is at fault
Traceback (most recent call last):
File "/home/data/miniconda3/envs/InPAC…
-
Hello,
I am currently using Salmon v1.2.0 which was build from source and is run on Ubuntu Linux
My objective is to obtain the proportions of different alternative spliced transcripts, which I …
-
i have fix the errors i asked 16 days ago ,but I get a new errors when do isoform reconstruction:
"""
Traceback (most recent call last):
File "/home/xxxx/anaconda3/envs/trim/lib/python3.7/multi…
-
Thank you for this helpful software.
I'm looking to look at the raw fastq reads for transcripts that came up as differentially expressed after quantification with Salmon. Is there a good way to li…
-
I recently ran Salmon by quasi-mapping-based mode and when I checked the salmon_quant.log file, saw that mapping rate was around ~%65-68 for all of the samples. Do you have any suggestions to improve …
-
Hi Brian,
I am using EVidenceModeler to combine different evidence and to have a final genome annotation.
I would like to include in my weight file:
- Ab initio gene prediction obtained from Au…
-
Hi
I have a pangenome graph with 5 genomes generated from minigraph-cactus.
I'm currently indexing with autoindex to use mpmap and rpvg.
When aligning RNA-seq reads should I align each set of…
-
Experiment: Single Cell RNAseq from 10x genomics
File Format: I have 2 Lanes (L001 and L002) and I also have for each of the lanes an R1 and R2 file
Reference Genome: Multiple HIV proviral sequences…