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Hello, it looks like the hybrid assembly approach outlined in the documentation is not supported for the 'ddrad' datatype in v0.19 and v0.20 (as is removed from the params file option). is this functi…
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### Description
I created a Maui Blazor Hybrid projects and I now want to publish this app in Release mode. But when I run the app in release mode, the entry point page "/", which is declared in a …
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Greetings,
I am interested in using this pipeline to remove haplotigs from a PacBio assembly. I am following the Pipeline Guide (Steps 1-3). I have a few questions regarding my output.
I assume…
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I have 20 de novo hybrid genome assemblies (ONT plus Illumina; flye plus pylon polishing) of different strains of the same species.
For an initial genome reference, we also have a high quality (T2T,…
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Hello,
I want to run the diploid portion of the pipeline to identify/remove alternative haplotypes if i want a haploid representation of the hybrid genome,should I run the `awk -f ${path_to_3ddna}/ut…
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Hi,
I´m wondering if there is a way to give idba a previous assembly from a subset of reads as a backbone/reference for an additional assembly of total reads. Would idba_hybrid provide such a functio…
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Hi Kangxiongbin,
Thank you for developing this impressive software. Utilizing both short and long reads for metagenomic assembly is a fantastic approach! However, I encountered some issues while us…
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Hi all,
Does it make sense to run arrow polishing after a hybrid assembly (w2rap-contiger/DGB2OLC) and Racon?
Thank you in advance,
Michal
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Suggest have a brutal check which rejects isolates where the nanopore/illumina data fail some kind of consistency test
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Good Day
Is this error a memory problem? On Linux cluster, (max 600GB mem, 4 nodes, 8 cpuspernode) I also get error code -11 on SPAdes 3.13.0:
== Error == system call for: "['/work/.apps/free72/…