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Hello!
I am so happy to have found this page - I have been trying to separate demultiplexed sequences using ngsfilter for so long, and this code has been so useful!
I have unfortunately hit a prob…
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### Description of feature
Hi, I have a couple of projects to analyse where the primers are degenerate and the last 3 or 5 bases have ambiguities that allow them to match the first 3-5 bases of the p…
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Hi @bachob5 @SantamariaMonica,
MetaCOXI is a very nice and useful database, thank you for developing it.
I would like to use the MetaCOXI_Seqs.fasta and MetaCOXI_Taxonomy_Metadata.tsv as input fi…
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Hello,
My issue is not a technical issue with mothur, rather a question related to my specific pipeline. I am currently using mothur v1.46.1.
I have 10 sites, where I have collected water sample…
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As per #87, it is clear ``amptk_mock1.fa`` has been processed differently from ``amptk_mock2.fa`` and ``amptk_mock3.fa``. It has no line wrapping, no species names, but does seem to have been left pri…
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## Update 2021-12-06
To join the nf-core community, please visit https://nf-co.re/join
The most important step is to sign up to the nf-core Slack, which is the hub of the community.
Once ther…
ewels updated
2 years ago
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Hi Mike! first of all thansk for the tutorial and clear info in your page!
I'm following both DADA2 tutorial (https://benjjneb.github.io/dada2/tutorial.html) and your pipeline too (https://astrobio…
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Hi there,
I have .fastq.gz files which have already been trimmed and filtered. I've followed the instructions to import the Silva_132_release database for 16S gene with sequences clustered at 99% ide…
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Dear benjjneb,
Thank you very much for this great pipeline!
We are currently developing the metabarcoding approach to characterize nematode communities in our soil samples. We would like to construc…
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**problem**: I have MiSeq PE metabarcoding fastq files from bacteria, but **no** mock community sequenced.
**I want** to validate my bioinformatics workflow.
**question**: as a partial solution, cou…