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Regarding the experimental approach, it seems that the authors did not further validate the transcriptomic analysis by qPCR or western-blot, which drastically lessen the reliability of the results.
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who in #Oncology-department knows #RT-qPCR and #Molecular-biology ?
 > sigmaContinuous, 1, sum, na.rm = T) :
> dim(X) must have a positive length
the assay was built like:
`assay
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Although libshark appears to be an external dependency, I cannot find any information about libshark anywhere, other than that it is retrieved from http://www.cs.cmu.edu/~robp/files/Shark.tar.gz
I am…
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16S rRNA amplicon sequencing only yields relative abundance and doesn't tell you anything about the actual number of (viable) bacteria.
I could fix this by doing qPCR on every sample using universal …
audy updated
10 years ago
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Create a script which analyses the Rapamycin effects on C2C12 differentiation and generates the bar graph used in Figure 1C. This should include a summary of the statistical tests used.
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Hi! Well, I think science is cool and I think genetics is interesting. =P
I like to code, a lot, I like to help and I was looking for an interesting project to put some of my free time in.
Working wit…
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`psmelt` returns a weird error when used with a phyloseq object with a single OTU.
``` S
library(phyloseq)
otus
audy updated
10 years ago
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@gregcaporaso noticed that if a mapping file header starts with a number (e.g. `16S.qPCR`), load_remote_mapping_file.py isn't able to export the file and errors out with the following message:
```
qi…
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function to upload many packages and post as gist
lapply(c("ggplot2", "psych", "RCurl", "irr", "car","Hmisc", "gmodels","qpcR"), library, character.only=T)