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Hi,
I try to run unicycler for a hybrid assembly and when I arrive to the step "Aligning read" after the step "SPAdes assemblies", the run is stuck to 100% :
Aligning reads (2018-09-06 18:17:33…
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Hello!
I've been using InSilicoSeq to generate mock communities for a project of my own to assess assembly quality (https://github.com/cimendes/LMAS).
To match the distribution of a real commun…
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Sometimes it makes sense to begin indexing a sequence from its center. For example with hyper-variable loop regions for T cell / B cell receptors or peptides presented by antigen presenting cells. The…
gszep updated
2 years ago
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Hello, i must admit that i am a novice at this, but bear with me, i am trying to configure this pipe line, it is not so clear where these configurations should be made.
In the manual you say that all…
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I run this:
wget ftp://ftp.ncbi.nih.gov/blast/db/FASTA/nt.gz
gunzip -dc nt.gz > nt.fasta
pathoscope2.py LIB -genomeFile nt.fasta -taxonIds 629395,2 --subTax -outPrefix LIB_for_Bacteria
stdout …
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I have tried to use Gibson to assemble two PCR fragments with a plasmid linearized by digestion with two restriction enzymes, but SYC does not find a compatible assembly. However, when I manually type…
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FreeGenes has a number of existing collections that may be valuable for us, such as the open reporters collection. We should review and see which ones may make sense to add.
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![snippy4](https://user-images.githubusercontent.com/5425346/54983830-431b1000-4fb6-11e9-906c-1ffcb1e3f703.png)
## Describe the bug
I have created a pipeline plugin and deployed it to an IRIDA insta…
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I have annotated several assmbled plasmids from several isolates using Prokka, and I am attempting to analyse them with roary. I installed roary using conda, added the gff files from the prokka output…
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## Summary of the feature idea
Introduce a "primer" element. Primers are short nucleic acid sequences that play a role in PCR reactions. For users, being able to create and view primers will facilita…