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Hello,
I apologise if this is not the right location to make this type of enquiry. I am completing my master's in diagnostics genomics and I have some concerns and questions that I wanted to reach …
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I am producing Nanopore libraries where I use two barcodes - one barcode at the front which represents a well on a 96 well plate and another barcode at the back which represents a specific plate. With…
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Hi I am running dorado-0.7.0 on my older sequence files where I converted fast5 files to pod5 and I used the SQK-LSK109 sequencing kit for these samples? Is there another kit I can choose from the has…
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Hello, do you have training data for R10.4, 4 and 5kHz? Or is that available via dorado? Or not at all?
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When I use guppy to basecall fast5 files with the --align-ref option selected, the required time increases by several hundred times. My fast5 files are approximately 100GB each. Is this normal? If it …
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### Ask away!
Hi! I am still trying to work out the kinks on running EPI2ME on HPC (need to get singularity permissions from admins), but I successfully ran some samples using our windows local lab …
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# Issue Report
## Please describe the issue:
I cannot get dorado installed correctly so that it will run. I keep getting the error "dorado: command not found".
## Steps to reproduce the issu…
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# Using bam files demuxed by Dorado with NanoPlot?
## Please describe the issue:
I ran rapid barcoding library (SQK-RBK114-24) on MinIon using barcodes 1-4. I basecalled the pod5 file and aligne…
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Hi,
I had optimised the batch size parameter (as noted in #660) using a test subset of my data which saw drastic improvements in 0.5.3 (Samples/s: 1.034746e+05 vs Samples/s: 3.626776e+06)
I have…
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Hi,
I generally parallelize dorado basecalling by generating a list of read_ids via `pod5 view`, and then split the read_ids and feed them iteratively to dorado via a batch script.
This has been …