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Fix bugs identified in first anospp production run
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Hello,
I'm wondering if anyone can assist with some issues I'm having re: locus assignment.
Some of my forward primers include ambiguous nucleotides (K, W, R, etc.) and some of the expected STR…
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### Description of the bug
I am recently running the viralrecon 2.6.0 on some sars-cov-2 sequences; I noticed that ivar.tsv output doesn't match with the snpeff. annotated vcf as well as the varian…
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Following our discussion with @nekrut , I am creating an issue to outline essential components for creating a barebones HIV (or other short RNA virus) genomic analysis pipeline.
Key steps for ampl…
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# Issue Report: std::runtime_error (core dumped) with Dorado 0.7.2
## The issue:
I have basecalled my data (short read amplicon data) with several versions of Dorado and keep on having the same (…
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Dear developers,
I am working with Illumina Miseq 300X2 data set of antibodies repertoires.
the amplicons are > then 600 bp, in the mergePairs function I added `justConcatenate = TRUE` because th…
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Hi,
I am trying to work with Porechop to demultiplex my data. The libraries are a bit complex, and I would really appreciate some help here.
We are doing microbiome work, and our hoping to do 16s…
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Hi there,
I am trying to run an amplicon-based (targeted panel) BD Rhapsody scRNA-seq data using kallisto/kallistobustools. Is there a way to map sample tags to cellular barcodes in order to ident…
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Not even sure if pangolin itself is the right repository to mention this issue/question or if it has been explained before.
While using some of our Illumina sequence data to compare it with our ON…