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### Description of feature
The current rna-seq pipeline, generates two bigwig files one each for each strand. it would be great if like chipseq pipeline, it generate only one bigwig files, would be h…
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### Need
As a user, I want to be able to visualise methylation. Although I'm probably not sure how.
One way would be to visualise it in IGV, either with bedMethyl or bedgraph (converted to bigW…
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## Describe the problem
When setting the color of a bar to fully red (RGB 255 0 0 with 100 Alpha) the bar color is set to white instead of red. This only happens with this exact value, changing a sin…
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It would be great to be able to fix current scale.
olegs updated
4 years ago
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**描述**
**重现Bug的步骤**
1. 第一步
仅启用rayui及bigwigs
2. 第二步
进入一个副本，比如紫罗兰监狱
3. 第三步
r…
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Following on from this week's concerns, it could be a good idea to have the option to generate plus & minus bigwigs for each BAM file, so can visualise strand-specific coverage over regions.
I can'…
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To call peaks from multiple replicates I first extended the reads, normalized the signal in each replicate, and then took the average signal at each base pair using my own code. I then did the same fo…
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I have a program that uses bx-python to extract data from a bigwig file for specific BED loci. For some reason certain chromosomes are sometimes skipped for having no data (BigWigFile.get_as_array( ch…
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I'd like to use pyBigWig to collect values at many intervals from many bigwigs, and I'd love to know what's performant.
1. is there overhead to opening a bigwig with pyBigWig? i.e. what's the runt…
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Hello,
I have some CAGE sequencing data from oil palm. The reads were mapped to the genome. I have alignment BAM file, and converted into BigWig. I have problem running quantifyCTSSs because I couldn…