-
Hi,
I am using hifiasm with parameter
hifiasm -o ./Hifiasm3_l3/Gon --primary -t20 --purge-max -l3 -s 0.35 --h1 ./HIC/Raw_Data/filtered_Insect_S118_L004_R1_001.fastq.gz --h2 ./HIC/Raw_Data/filtered…
-
Hello, I hope you are well!
I'm able to download a specific NCBI Assembly via `bactopia --accession [accession]` -- is there a way to do so with a list of NCBI Assembly accessions (i.e bactopia --…
-
To whom this may concern,
I ran the "run_example.sh" test and got the following error message on a Linux cluster:
ERROR: Component "Interleaver" (sample:Modified) failed, giving the following er…
-
**Description**
I was trying out Bactopia with some uncompressed FASTQs from ONT, when the run crashed during the gathering stage. It seems like Bactopia is assuming that ONT reads are properly gzip'…
-
```
$ metawrap assembly -1 CLEAN_READS/ALL_READS_1.fastq -2 CLEAN_READS/ALL_READS_2.fastq -o ASSEMBLYOUT
metawrap assembly -1 CLEAN_READS/ALL_READS_1.fastq -2 CLEAN_READS/ALL_READS_2.fastq -o AS…
-
I am trying to run ipyrad on a set of fastqs that I previously demultiplexed. I am receiving this error for all the samples: "No PE fastq pairs detected based on filenames, assuming SE data."
I add…
-
I using the latest kmc code but i can't count kmer on fastq file. It work on fasta
```
$cat r1_test.fq
@0|Chromosome|4051100|4051286/2 BX:Z:CGACACGGTTTGGGCC
AAACCCAACCAC
+
FFFFFFFFFFFF
$kmc …
-
Hello. I am currently trying to realign the raw data from this paper using the kallisto-bustools pipeline. I am able to specify custom technology by demarcating which read and the start and end positi…
-
Hello,
I only have one fastq file for the sample. When running the amplicanPipeline function I get an error that my forward file is blank, but my forward file column contains the name of my fastq f…
-
### Is your feature related to a problem?
When using a aligned BAM file as input, wf-human-variants does take the alignment as it is, without doing a re-alignment. Of cause this makes a lot of sens…