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1. SMART link user guide: https://www.pacb.com/wp-content/uploads/SMRT_Link_User_Guide_v10.1.pdf
2. Pacific Biosciences Glossary of Terms: https://www.pacb.com/wp-content/uploads/2015/09/Pacific-Bios…
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Hi all,
I've been trying to run HINGE using Circular Consensus Sequencing reads that are obtained after a Sequel run. As expected the number of reads decreases drastically due to CCS, but on the ot…
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The field will be used to specify the sequencing platform applicable for the application.
It will be located next to the field "prep category".
"Platform" needs a drop down list with the following…
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Hello,
Firts of all, I would like to express my appreciation for the development of AA. I have been using AA extensively, and it has been a crucial tool in my research.
I am planning to use Hi…
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Hello!
I'm having a slight issue with the annotations generated by EGAPx. When I translate the CDS into a protein sequence, I'm seeing a lot of premature stop codons. This is a species where I am s…
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Hello
I need some suggestions in simulating complex structural variants in Hifi reads file from PacBio
https://downloads.pacbcloud.com/public/dataset/HG002-CpG-methylation-202202/m64011_190830_22…
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I am very interested in the development of strainberry, especially as it applies to metagenomics with PacBio HiFi data. I wanted to draw your attention to a new PacBio dataset I recently uploaded to N…
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Hello @rrwick,
I experienced some poor mapping performance with Minimap2 for reads simulated using the pacbio2016 (only changing depth, mean sequence identity, all other settings as default). I notic…
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Thank you all for developing CoRAL. I have a question regarding the error message during infer_breakpoint_graph step and wonder if there are any suggestions you could kindly have:
Error message:
T…
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Hi,
I am trying to load a gtf file but run into an error I could not solve using the importGTF function:
>
> Step 1 of 10: Checking data...
> Step 2 of 10: Obtaining annotation...
> imp…