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I've been testing tombo 1.4 on some of our DRS data and I'm finding that the resquiggle step is taking a *long* time to complete and it using a *lot* of resources.
The dataset I'm running it on is …
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Hello,
I would like to simulate ONT rolling circle amplification data. Can the NanoSim software be used for this purpose?
Best wishes,
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Hello,
I received the following error output when running DNAscent detect:
```DNAscent detect -b Tmet_079_pass_mapped_R_index_filt_header.sorted.bam -r /hg38.chrXYM.fa -i Tmet_079_reads_pass_R.…
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I have nanopore long-reads from tumor (tumor only)
I also have SNP/indel variant calls from the same sample from illumina in both germline (normal) and tumor data
I want to try and phase the tumou…
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### Description of bug
Hi, I was running hybrid assembly with Illumina short reads + PacBio ccs. My short reads are merged into a single fastq after QC pipeline, consisting of both paired and orpha…
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2017.3.24 上午
====================
基因组
2017.3.24
====================
1.什么样的物种适合全基因组测序?
能被高引用的,才可能高分文章。
天山雪莲,从稀有、中药角度,老外估计不喜欢。
如果有天山雪莲的体外培养系统,做成耐寒的模式生物,则有可能发高分。
end9:45
2.技术流、思路哪个重要…
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Hi - We are trying to use NanoMod to call out EdU bases in Nanopore sequences. Since Albacore is no longer supported, we use Guppy to do basecalling. We used the "fast5_out" command during basecalling…
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Hello hifiasm team,
I am trying to use hifiasm on simulated HiFi and Nanopore reads (so both datasets are haploid, since they came from published genome assemblies. As I read in the documentation,…
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The developer group agreed to pre-populate with "Illumina MiSeq" since that is the most frequently used instrument.
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I am trying to run a long read pipeline with ONT long reads data for mouse models using Terra platform.
I was able to run https://github.com/broadinstitute/long-read-pipelines/blob/kvg_guppy_cpu/wd…