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Hello!
Could you please provide me with the information on your plan about adapter trimming incorporating to the Dorado. It is the feature I rely on in my workflow and it was that I used in guppy. No…
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I am probably doing something dumb but after I download and extract the dorado folder , when I try to click on the dorado application it opens for half a second and then immediately closes (Not enough…
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Hi all,
I have performed dorado modified basecalling on my data, obtain my assemblies and performed a modkit pileup on them to have my methylation data.
I have filtered my data to have output as f…
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Calling the scheduler with the following command:
> dorado-scheduling "${SKYMAP_DIR}/${num}.fits" -o "${SCHED_DIR}/${num}.ecsv" -j --mission=uvex --exptime="${texp} ks" --duration="${DURATION}" --r…
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# Issue Report
## Please describe the issue:
I have a DNA sample sequenced with R9.4.1 that I wonder if I could do duplex basecalling on it or not.
According to the current model selection so…
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# Issue Report
## Please describe the issue:
I am performing basecalling with alignment on a MacBook M2 pro device. I have a pod5 files with ~4000 reads (~200 MB) which each take 5-6 minutes for…
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Hello,
I've been working with both the integrated Dorado basecaller in MinKNOW and its standalone version one dataset. I have a couple of questions I was hoping you could help me with:
1. Could yo…
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Hi,
I got Nanopore full-length transcriptome data from a **PromethION 2 Solo** machine.
For the library preparation, we used the **Native Barcoding Kit 24 V14 (SQK-NBD114.24)**.
**Can I use …
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Hello,
I have a set of unaligned BAMs seperated by barcode given to me by dorado. I was wondering if it would be possible to use these with the `--barcoded` flag to get a summary of each barcode, a…
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Hi, we have been running duplex basecalling without issues in v0.4.x, but all versions of v0.5.x we have tested (i.e., .0, .1 and .2) throw the same CUDA out-of-memory error (see below). This error ha…