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Hello,
I am using Clair3 for variant calling on amplicon sequencing data from a minION, generally with a fair amount of success. However, for one particular amplicon, I get a large number of false ne…
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Hello,
I am trying to use the tool for my long-read sequencing data from the ONT MinION platform. I had mapped the reads using minimap2 and had given the same SAM file as input, however, the proces…
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# Issue Report
## Please describe the issue:
I have a multi-GPU system and I am unable to assign the correct GPU to use with the --device flag. The NVIDIA GPU are listed as device 0 so I have t…
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### Operating System
macOS
### Other Linux
_No response_
### Workflow Version
wf-basecalling v1.1.2.
### Workflow Execution
EPI2ME Desktop application
### EPI2ME Version
v5.1.8
### CLI comma…
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# Issue Report
## Please describe the issue:
We found a lot of mutations in the base called RNA reads using Dorado 0.5.3, the RNA is the product of in vitro transcription and the reads are mappe…
yul96 updated
7 months ago
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Hi, can you add the option to use the latest clair3 model for germline variant elimination? Thank you.
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### Ask away!
Dear Team,
I have recently started investigating CNV (Copy Number Variation) issues using nanopore hg002 data in my research workflow, with the hg38 reference genome. During my analysi…
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Hi @marcus1487,
I am running into an issue that I was hoping you could provide some insight into:
I have a dataset from a P2 sequencer that was basecalled and aligned using ```dorado``` v0.5.1.…
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I have a bunch of BAM files called with `dorado` and like to feed these into this workflow.
Is it possible to omit basecalling step and start later e.g. with alignment?
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Hello,
When I run the detect function, I get about halfway through the calling before getting the error "HDF5-DIAG: Error detected in HDF5 (1.8.14) thread 140035900785152:ec" that causes the read t…