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Hello,
I have been running metaSPAdes v. 3.14.1 on MacOS Mojave for Illumina HiSeq paired-end 2x150 data (about 25 million reads total).
Everything runs smoothly until it reaches the K55 stage …
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Hi,
I have some questions about the fixref plugin :
1) Looking at the code, I can see that "TOP-compatible" and "BOT-compatible" outputs in the logs are not counts but can only take 0 or 1 value…
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Hi.
I'm trying to analyze data from viruses (in illumina paired end fastq format), with a size of 100 MB for the sum of R1 and R2, in a machine with 16 cores and 30 GB of ram memory.
In previous s…
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**Describe the bug**
Reading from SRR25144788_MN908947_3_bwa-mem_sorted.bam
Minimum Read Length based on 1000 reads: 50
Segmentation fault (core dumped)
**To Reproduce**
ivar trim -i SRR251…
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Hi @alexdobin
I used Star aligner for a while and I really appreciate the tool you developed.
Recently, I use to trim the RNAseq short and long reads with the fastp tool https://github.com/OpenGene…
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Hi ndierckx,
I have been trying to use NOVOPlasty to assemble a mitochondrial genome from illumina PE short read data, and despite many runs tweaking all parameters I have only managed to have the pr…
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Hi, how to deal with multiple variants in a small region, is there a way to combine them to one variant by Pisces or other tools? Attached file describes the situation we find about EGFR exon 19 delet…
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Hi! Thank you for your great tool!
I have the same question as [here](https://github.com/Illumina/ExpansionHunter/issues/35). Could I use ExpansionHunter for samples prepared with PCR-not-free protoc…
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Hi,
Thank you for developing BrownieCorrector- I am grateful that you are addressing the issue of poly-nucleotide stretches in Illumina data and am excited to try the software.
When I tried a qu…
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Dear Mahul, I intend to use SVMU for calling variants between a reference and a newly sequenced strain of Drosophila. I have the contigs and also a scaffolded assembly for the same built from Illumina…