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I am trying basecalling and after feeding the prompt with the commands to run my basecalling I am getting the CUDA device requested but no devices found.
By reading on the Dorado recommendations I…
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Dear Authors,
When playing with your provided fast5, we occasionally observed several reads are continuously reported as "no_map" in the RU log even with many iterations and long lengths (>30, >7kb).…
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Hi, I would like to ask for help in solving my problem with flye.
After installing flye in my ubuntu terminal (to be more precise, I created an environment with conda and installed flye there), I f…
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Hi,
To test Dorado vs Guppy for direct RNA basecalling, I am basecalling ~1.3M direct RNA reads using Dorado 0.3.4 on a cluster with 4 x Tesla Volta V100 32GB GPUs.
Strangely, for a library wi…
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Hi there,
It's usually a bit "annoying" to choose the correct medaka model for genome polishing based on the provided guppy model. However, the newest MinKnow software prints the basecalling model …
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Hi,
Some weeks ago, we did a sequencing run to test ReadUntil without reference mapping and basecalling. Everything went fine and I could recognize a peak at 500bp as expected. But after 3 hours I …
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Hi,
I'm recalling some data in an only CPU server with Guppy 4.4.1 using the res_dna_r941_min_crf_v031 model provided here, using 32 threads, but it's taking such a long time. Is a GPU needed for thi…
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Hello,
When running dorado duplex I got an error: 3607421 Segmentation fault (core dumped) .
Data was produced by a promethION and kit SQK-LSK114.
basecall model - dorado-0.4.2-linux-x64/bin…
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Hi when trying the new auto model selection I get the following mesage
```
./dorado basecaller hac Run12/ > Run12.bam
[2023-12-06 15:51:37.035] [error] Unknown flowcell_product_code:
```
Tha…
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Hi,
When I running `dorado duplex ` I got error : segmentation fault (core dumped). Could you help me to solve this problem?
My data was produced by miniON in `5khz`, and kit is `SQK-NBD114-96`
I…