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Hi everyone,
I need a little help to deal with my assembly. I usually use Spades but since I found that Unicycler could improve my assembly, I always perform the two separately and Unicycler get the…
fetyj updated
5 years ago
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If i run without parameters, i get the usage as expected.
then if i run with parameters
scaff10x -nodes 30 funestus_redbean.purged.fa bamtofastq_S1_L000_R1_001.fastq.gz bamtofastq_S1_L000_R2_001.f…
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Hi,
I am running a de novo hybrid assembly with illumina PE and pacbio long reads, and i'm facing a disk space issue. The intermediate files that MaSuRCA writes is consuming all my disk space and t…
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Hi Ryan,
Thanks for writing Unicycler, it is a very useful tool.
I ran a hybrid assembly using conservative mode (same result for normal mode) and I obtained 3 contigs, as follows:
>1 length=41…
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## Is your feature request related to a problem? Please describe
At the moment there is an input option to use only [short reads](https://nf-co.re/mag/usage#direct-fastq-input-short-reads-only).
…
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HI,
I am working on a hybrid assembly for a 3.2G allotetraploid plant genome:
1. Illumina assembly with [w2rap-contigger](https://github.com/bioinfologics/w2rap-contigger).
2. [DBG2OLC](https:/…
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Do you recommend any QC on the short and / or long reads before submitting to OPERA-MS for the hybrid assembly of metagenomic samples?
For short reads that would include removing adapters, trimming…
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Thank you for this parsing tool! I found it very helpful.
Wanted to share a notebook that I made, which parses data with fdapa then makes a plot. Similar to multiqc but allows for better control …
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HI,
I am working on a hybrid assembly for a 3.2G allotetraploid plant genome:
1. Illumina assembly with [w2rap-contigger](https://github.com/bioinfologics/w2rap-contigger).
2. [DBG2OLC](https:/…
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Hi @rpetit3 ,
Thank you so much for upgrading `Bactopia` to v2 which included supports for only Nanopore reads! We have been waiting for a very long time!
May I know is there a tutorial part in …