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Hi,
I incidentally poked over your project and I wonder why you keep track of `Average read depth` and of `Observed insert size`. The former would be better replaced with `Median read depth` and th…
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Heyhey, could you add the following biography to the website? I've also attached my picture to the post.
Thanks a lot!
### Lancelot Seillier, M.Sc.
Scientific Researcher / Bioinformatician
…
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Hi,
First,
I am wondering the step:
` Finally, the HiFi and short reads were combined to polish the ONT assembly. `
What software did you use to polish the genome?
Second,
what are the sequenc…
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It is very useful to know Ori/Ter in draft genomes.
However, is there a way to reorder multiple non-overlapping contigs then, such as from a WGS Illumina sequencing experiment yielding a 30-100 con…
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Hi,
This issue isn't really an issue per se, but more a question about the status of `lofreq3`.
I use `lofreq` version: 2.1.5 in an analysis pipeline for SARS-CoV-2. I like updating to the lat…
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Hi there, it is a great work of Bacmethy. I'd like to know if Bacmethy only supports Single Molecule Real-Time(SMRT)Sequencing data or it is compatible with Illuminasequencing data ?
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### Is there an existing issue for this?
- [X] I have searched the existing issues
### Description of the Bug/Issue
I'm working on validating this pipeline on samples sequenced on both Illumina and…
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Hello,
I'm Sebastian, working on my MSc thesis, aiming to create a knowledge graph of the wastewater treatment microbiome. We're using Netcomi for constructing Microbial Association Networks (MANs)…
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I am using "MarkDuplicates" (2.18.2.2) to remove duplicates from my sequencing datasets coming from NovaSeq. From Picard documentation it suggests the OPTICAL_DUPLICATE_PIXEL_DISTANCE to be 2500 for …
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**Bug Description**
I have recently installed an Illumina iSeq-100 benchtop sequencer in my lab, and it was announced allready in 2018, that there will be a 2x 250 bp sequencing cartrige available so…