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Hello,
I am using m6ANet to detect m6A modifications in RNA and recently swapped over to the new nanopore RNA004 chemistry/kit. Because of this, my pipeline uses ONT's new basecaller, dorado, and I…
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Hello,
I'm trying to fine tune a model with large repeat expansions (>500 hexamer repeats), where the standard model hallucinates biologically impossible repeats. The issue is that the number of re…
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### Operating System
Windows 11
### Other Linux
_No response_
### Workflow Version
1.1.3
### Workflow Execution
EPI2ME Desktop (Local)
### Other workflow execution
_No response_
### EPI2ME V…
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https://mp.weixin.qq.com/s/cPsR1i49__zQZmTcLDmg0g
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Dear developer:
I am trying to assemble the genome using about 15-20X nanopore data and about 15X illumina genome. The genome we are going to assemble , I would like to ask if this is feasible. I don…
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HI, sorry for bothering you again;
When I tried with the actual data
I just changed the line 178 with the right dir containerOptions = '-v /home:/home -v /autofs/tong1/LSX/lishuxian/lishuxian/202403…
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I attempted to use the tool with the BAM file generated from demo data and encountered an error. Could you kindly provide assistance with this?
```
(venv) (base) kamada@Lalithas-MacBook-Pro Nano…
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Hi, according to the paper, it says it support RNA-seq data and long-read sequencing data. I am wondering whether it can do HLA typing only based on long-read RNA-seq data (Oxford Nanopore data)?
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Hi everybody.
We have a unaligned nanopore bam file with MM/ML tag. We use minimap2 and samtools to align the bam with reference.fa to get the sorted bam, and then run the modkit pileup to try to g…
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Hi,
I have setup a playback run using MinKNOW v5.8.13, minION MK1B, selected all default options and using readfish for basecalling. I can't figure out from the documentation where is the output. …