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Hi,
Is there a way to run dorado on a subset of/specific pod5 files? If not, can you please add this similar to the --input_file_list parameter from guppy.
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Hello, I installed dorado by dragging the bin and lib folder into the bin and lib folders of my VM (Ubuntu). When i type in the following comand that happens:
**dorado basecaller -x "cpu" rna002_7…
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I have been trying to assemble a 10Mb genome with uncorrected nanopore data (3-4 chromosomes expected). We have a lot of data, is that the reason Flye fails at the end?
[2019-06-22 11:00:05] INFO: …
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Hi
Keeping track of all the ONT version numbers can be a bit tricky. Maybe it would be relevant to align the names from the guppy basecaller with medaka. Now R10 data would need the dna_r10_450bps_…
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Is it possible to store base-called sequences in the FASTQ format into POD5 files using Dorado, similar to how it was achievable with FAST5 files using `guppy_basecaller --fast5-out`? Additionally, I …
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Hello,
What is the best format to use when rebasecalling?
I read that .BAM files are sequence alignment maps whilst fastq usually comes from sequencing instruments.
So if I just basecall and not…
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I'm working on a set of fast5 files that i would like to do basecalling on.
So far i've converted the fast5 files one by one to pod5 files using the pod5 software. If there is a clever way to way t…
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### What happened?
Hello,
There is no medaka model for the R10.4.1, 260bps, super accurate basecalling. What should I do?
### Operating System
Windows 10
### Workflow Execution
Command line
…
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Hi, everyone
when I run `tombo preprocess annotate_raw_with_fastqs` get no results. the full command of a small example is
```sh
# directory fast5/ stores the read b82c9dc6-29df-4807-8c88-9911…
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I have some historical ONT runs that were basecalled with these models:
run | sample | base_call_model
-- | -- | --
4031P | A-1 | 2021-05-05_dna_r9.4.1_promethion_384_dd219f32
4092P | A-1 | dna_…