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# Issue Report
## Please describe the issue:
I want to estimate poly-A during basecalling for direct RNA-seq data and have bam file with adapter-trimmed reads as output. But using option --estim…
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Hi,
i run modcall with modified.bams that have MN & ML tags however the resulting don't have `|` at the genotype position meaning the results were unphased. here's command that i used for modcall an…
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Hi there!
I have some methylation data that I generated using the Oxford Nanopore P2 Solo. I currently have 2 samples with 1 biorep each (human cancer cell line, non-resistant parental line and one…
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# Issue Report
## Please describe the issue:
I am using the Native Barcoding 96 kit with R10.4.1 5kHz, and I'm observing a huge difference between ont-dorado-server and dorado standalone. It's ~…
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# Issue Report
## Please describe the issue:
I want to correct reads.
## Steps to reproduce the issue:
```shell
cd /lizardfs/guarracino/tools/
wget -c https://cdn.oxfordnanoportal.com/softwa…
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Hi Saskia!
Hello from Canada!
I am using Decona to genotype some amplicon data and I have run into some trouble.
First, I was able to run the example data with no problem.
But, with, my …
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Hi,
For my duplex-called reads, I get
```
$ A=$(samtools view all.bam | grep -c 'dx:i:0')
$ echo $A
622019
$ B=$(samtools view all.bam | grep -c 'dx:i:1')
$ echo $B
304276
$ C=$(samtools view…
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**Copy and paste the exact command you tried to run**
```
conda activate pychopper_env
pychopper -k PCS111 -t 20 $lr_dir/${sample}.fastq $lr_dir/${sample}_py.fastq
conda deactivate
# Ac…
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Hi Dorado team,
I have two questions about the mRNA modifications in the new dorado release 0.7
1. For the pseU modification, is it for pseudouridine or N1-methylpseudouridine?
2. Is it pos…
yul96 updated
5 months ago